Learn how to obtain and analyze UBM images with a validated and comprehensive protocol

Analysis Protocol

 

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Anterior segment ultrasound image analysis is performed using ImageJ, an open source, publicly available toolbar.  

 

 

 

 ImageJ measurement of central corneal thickness

ImageJ measurement of central corneal thickness

This image processing program allows for measurement of clinically relevant structures such as the central corneal thickness, and also for measurement of structures that are not typically measured, such as the length of a ciliary process.  

 

 
 ImageJ measurement of ciliary processes

ImageJ measurement of ciliary processes

 

Some structural findings related to anterior segment anatomy (in particular the ciliary body) have unknown clinical significance. Unfortunately, current literature lacks prospective studies relating quantitative objective anterior segment structural data and long term outcomes. We hypothesize that the size and orientation of these under-studied anterior segment structures may be clinically meaningful. By use of a prospective analysis system, we hope to identify these significant structures. 

 

Our measurement protocol has been put to the test in terms of reliability and reproducibility and has undergone multiple revisions so that clinicians worldwide can share a common protocol. 

Analysis Video 1: Complete analysis protocol using ImageJ

References:

1. Pavlin CJ, Sherar MD, Foster FS. Subsurface ultrasound microscopic imaging of the intact eye. Ophthalmology 1990; 97:244–50.

2. Pavlin, C. J., MD, & Foster, F. S., PhD. (1995). Ultrasound Biomicroscopy of the Eye (1st ed.). New York, NY: Springer.

3. Silverman RH. High-resolution ultrasound imaging of the eye - a review. Clin Experiment Ophthalmol. 2009 Jan;37(1):54-67. doi: 10.1111/j.1442-9071.2008.01892.x. Epub 2008 Dec 9.

4. Sakata LM, Lavanya R, Friedman DS, Aung HT, Seah SK, Foster PJ, Aung T. Assessment of the scleral spur in anterior segment optical coherence tomography images. Arch Ophthalmol. 2008 Feb;126(2):181-5. doi: 10.1001/archophthalmol.2007.46.